WebNov 21, 2013 · Stability of luciferase expression in the absence of selective pressure. (A) The Ppy RE9H luciferase signal of a T. brucei s427 clone parasite maintained in the presence or absence of 1 µg ml −1 of puromycin. (B) The Ppy RE9H luciferase activity expressed by a T. brucei GVR35 clone. No significant decrease in bioluminescence was … WebSchematic illustrating integration of linearized pTRIX2-RE9h into the T. cruzi genome by homologous recombination. Integration was confirmed by Southern blot of BamHI (B) and XhoI (X) digested ...
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