Methylphosphonate oligo
Web1 jun. 1993 · A novel method of deprotecting methylphosphonate oligonucleotides was developed from these studies. The method incorporates a brief treatment with dilute ammonia for 30 minutes followed by... Web11 mei 1993 · A novel method of deprotecting methylphosphonate oligonucleotides was developed from these studies. The method incorporates a brief treatment with dilute ammonia for 30 minutes followed by addition of ethylenediamine for 6 hours at room temperature to complete deprotection in a one-pot format.
Methylphosphonate oligo
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WebPhosphonocarboxylate oligonucleotides are recently-developed modifications that can be incorporated using “phosphoramidite-like” monomers. 1,2 The monomers, shown in Figure 1 are formally called “phosphonamidites or phosphinocarboxylic acid amidites” because they contain a carbon atom attached to the phosphorus atom instead of a protected oxygen. Web27 aug. 2024 · Methylphosphonate bonds are non ionic nuclease resistant linkages. Methylphosphonate/RNA duplex are not recognised by RNaseH. Length: From 5 to 40 bases Synthesis scale: 40 nmol - 200 nmol - 1000nmol Modifications: More than 300 modifications available Compatible Purification: RP-HPLC Quality Control: MALDI-TOF MS
WebIn order to be useful as therapeutic agents, oligonucleotides must possess a number of properties. These include:1.Resistance to enzyme degradation; 2.The ability to enter target cells; 3.A lack of interference with normal DNA and RNA processing enzymes; and 4.The ability to bind to a target and alter its expression in a sequence-specific manner. Web301 Moved Permanently. nginx
Web1 dec. 1997 · A novel method of deprotecting methylphosphonate oligonucleotides with ethylenediamine was developed and found to be superior in yield of product by as much as 250%. 61 PDF Internucleotide phosphite sulfurization with tetraethylthiuram disulfide. Phosphorothioate oligonucleotide synthesis via phosphoramidite chemistry H. Vu, B. … Webneutral analogs, methylphosphonate oligo-mers (Figure 2-1a) and phosphoramidates (Figure 2-1b) have been readily prepared on solid supports. Although these analogs have an increased resistance to nucleases, they show a lower RNA binding affinity than natural phosphodiester-based parent se-quences. This reduced affinity has been at-DNA mRNA ...
WebCustom Oligonucleotide specifications. Length: From 5 to 139 bases. Synthesis scale: 10 nmol • 40 nmol • 200 nmol • 1000 nmol • 2.5 μmol • 5 μmol • 10 μmol • 20 μmol 1. Backbone: DNA, RNA, LNA®, 2’O-Me RNA, 2’-O-MOE, PNA 2, phosphorothioate, methylphosphonate and all linkages. Modification:
WebAnnotate RNA to peptide crosslinks in MS/MS spectra. The command line parameters of this tool are: fmm-101 notifier manualWeb20 jan. 2003 · The LNA methylphosphonate oligonucleotide ON2 ( Table 1) was synthesized in 0.2 μmol scale on an automated DNA synthesizer using amidite 2 and commercial DNA phosphoramidites ( tert -butylphenoxyacetyl protected G and phenoxyacetyl protected A and C). fmm-101 notifier datasheetWebOligonucleotides as Potent Human Nucleotide-Containing Phosphorothioate ... 42, 47, 48, 50, 51, 53), methylphosphonate ONs (15, 32, 46), or phosphoramidates ONs (6, 12, 14), in order to improve the stability of the antisense ONs against nucleases. Among these modified ONs, PAS ONs have been fmm130 firmware errataWebDimethyl methylphosphonate ≥98% Synonym (s): Dimethoxymethyl phosphine oxide, Fyrol DMMP, Methanephosphonic acid dimethyl ester, NSC 62240, Reoflam DMMP Linear Formula: CH3P (O) (OCH3)2 CAS Number: 756-79-6 Molecular Weight: 124.08 Beilstein: 878263 EC Number: 212-052-3 MDL number: MFCD00008349 PubChem Substance ID: … greenshades accountWebA novel, structurally defined, and homogeneous oligodeoxynucleoside methylphosphonate (oligo-MP) neoglycopeptide conjugate, [YEE(ah-GalNAc)3]-SMCC-AET-pUmpT7, has been synthesized. The linkage between the carbohydrate ligand and the oligo-MP is a metabolically stable thioether. green shades accountingWeb15 aug. 2016 · For instance, oligos built up with methylphosphonate, phosphorothioate and α-anomer units (see fig. 1) lead to molecules that are resistant to DNases. Acridinelinked oligos exhibit an increased affinity for the target sequence due to the intercalation of the dye into the oligo/RNA duplex. greenshades 1099 filingWebNucleic acid oligonucleotide sequences are disclosed which include amplification oligomers and probe oligomers which are useful for detecting multiple types of human papillomaviruses (HPV) associated with cervical cancer. Methods for detecting multiple HPV types in biological specimens by amplifying HPV nucleic acid sequences in vitro and … fmly mgt/coe