Heparg differentiation

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August undefined, 2024

WebFully differentiated HepaRG™ cells are the hepatic cell line of choice for in vitro study in toxicology and drug trials. They are derived from a hepatoblast-like progenitor (HepaRG … Web1 aug. 2024 · In contrast to many hepatoma cell lines, differentiated HepaRG cells exhibit specific liver functions resembling those of adult primary human hepatocytes, including abundant CYP450 enzymes, such as CYP1A2, CYP2B6, CYP2E1, and CYP3A4, phase II enzymes, drug transporters, and ligand-activated nuclear receptors [3], [4], [5], [6], [7].

The importance of transporters and cell polarization for the …

Web9 apr. 2014 · Differentiation of HepaRG Progenitors Into Cholangiocytes HepaRG-HB were treated for 2 days with IL-6 (10 ng/ml), then for 2 days with sodium taurocholate hydrate … WebThe cytochromes P450 (CYPs) represent a large gene superfamily that plays an important role in the metabolism of both exogenous and endogenous compounds. We have reported that the testis-specific Y-encoded-like proteins (TSPYLs) are novel CYP gene fisher price nativity sets for children

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WebHepaRG cells have superior expression levels of key uptake and efflux transporters compared to other cell lines and expression levels closely resembling those of human hepatocytes (Figures 5 and 6). They also … Web31 okt. 2024 · HepaRG is an original human hepatoma cell line, acquiring highly differentiated hepatic features when exposed to dimethylsulfoxide (DMSO). To search … WebA) Compared to the 2D plated cells the proliferation of the low-density progenitor cells in the hydrogels was remarkably slower. B) At high-density cultures, the differentiated cells did not... can aluminium oxide be reduced using carbon

DMSO-free highly differentiated HepaRG spheroids for chronic …

WebOur results indicated that the enhancement of hepatic functions in 3D-cultured HepaRG cells was induced by selective hepatocyte differentiation and accelerated hepatocyte … Web12 feb. 2024 · HepaRG is a bipotent stem cell line that can be differentiated towards hepatocyte-like and biliary-like cells. The entire cultivation process requires 1 month and … can aluminium windows be paintedWeb22 jul. 2024 · The well-characterized HepaRG cell line in 2D culture depicts a closer phenotype to PHH than HepG2 cells concerning the expression of phase I and II enzymes, transporters, and nuclear factors.... fisher price nature touch

"Web23 jan. 2024 · HepaRG and HepG2 cell lines were used as references. It should be noted that these cancerous cell lines are not ideal representation of hepatocytes, but they are widely used as hepatic model cells. During differentiation albumin was first detected at the endodermal stage and its level was gradually increased at subsequent stages ( Fig 1B ). " - Heparg differentiation

Heparg differentiation

Determination of in vitro hepatotoxic potencies of a series of ...

Web一站式科研服务平台. 学术工具. 文档翻译; 收录引证; 论文查重; 文档转换 WebHepaRG™ cells exhibit many characteristics of primary human hepatocytes and are essentially a single donor. These features allow users to obtain physiological relevant results for metabolism-based drug-drug interactions without the concern of donor variability and limited lot sizes that come with relying on donor tissue.

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Web25 jan. 2024 · The HepaRG cell line is a human bipotent progenitor cell line that can be differentiated into HLCs and biliary epithelial cells [ 19 ]. Whole-genome expression profiling showed that HepaRG cells are remarkably similar to human hepatocyte populations [ 52 ]. Web26 mei 2024 · Further, NRF2 inhibition drives the trans-differentiation of HepaRG cells, a human liver-derived cell line that shows phenotypical markers of hepatocyte-like and cholangiocyte-like cells 22.

Web12 feb. 2024 · HepaRG is a bipotent stem cell line that can be differentiated towards hepatocyte-like and biliary-like cells. The entire cultivation process requires 1 month and relies on the addition of 2% dimethyl sulfoxide (DMSO) to the culture. WebHepaRG® Differentiation Medium Supplement with antibiotics. For the use of HepaRG® cell line, as supplement for differentiation medium, according to BIOPREDIC …

Web13 nov. 2002 · To elicit the factors that determine the HBV susceptibility of HepaRG cells, we compared the infection efficiency on poorly and highly differentiated cells. To this purpose, we modulated the differentiation state of HepaRG cells by adding DMSO up to a final concentration of 2% and determined the accumulation of the CYP 3A4 transcripts … Web27 okt. 2024 · Data Availability Statement. The raw data can be assessed from ENA: In vivo liver: PRJEB35350/ERP118386, PHH: PRJEB23590/ERP105351, iPSC-HLC: PRJEB23620/ERP105382, 3D liver microtissues: PRJEB24482/ERP106310, HepG2: PRJEB24466/ERP106294 and PRJEB24464/ERP106292, HepaRG 3D: …

Web11 nov. 2024 · HepaRG cells show high plasticity in 2D culture, being able to differentiate into biliary and hepatocyte-like cells after exposure to DMSO (Aninat et al. 2006). Our …

WebFurthermore, DMSO is cytotoxic at the concentrations used; indeed, the original description of HepaRG cell differentiation notes that the majority of the cells die in the presence of 2% DMSO and it is only the small number of surviving cells that go on to differentiate (Gripon et al. Citation 2002). fisher price nativity walmartWeb1 jan. 2010 · The HepaRG cell line, which is a human bipotent progenitor cell line capable to differentiate toward two different cell phenotypes (i.e., biliary-like and hepatocyte-like cells), has been established from a liver tumor associated with chronic hepatitis C. can aluminum alloy be weldedWeb18 jan. 2024 · 1,2-unsaturated Pyrrolizidine Alkaloids (PAs) are secondary plant metabolites that occur as food contaminants. Upon consumption, they can cause severe liver damage. PAs have been shown to induce apoptosis, to have cytotoxic and genotoxic effects, and to impair bile acid homeostasis in the human hepatoma cell line HepaRG. The major mode … can aluminium foil go in the ovenWeb4 jan. 2024 · HepaRG differentiation medium was prepared by adding HepaRG Differentiation Supplement (Lonza, cat# ADD721C) to 500 ml of William's E medium. Both were supplemented with 2 mM Glutamax and antibiotics (100 units/ml penicillin G sodium, 100 µg/mL streptomycin sulphate). fisher price nature\u0027s touch cradle swingWeb10 dec. 2024 · Differentiated HepaRG cells show similar or higher gene expression of transporters such as multidrug resistance proteins (MRP1 and MRP2) and organic anion transporting polypeptide (OATP) 2B1 as well as substantial expression of MRP2, MRP3, BSEP, and P-glycoprotein compared to primary human hepatocytes [ 10, 15, 22, 23 ]. can aluminum and steel be welded togetherWebIn the present study, we evaluated the effect of alcohol on hepatocyte differentiation in relationship with changes of iron metabolism in HepaRG cells. This unique bipotent human cell line can differentiate into hepatocytes and biliary epithelial cells, paralleling liver development. Results showed that alcohol reduced cell viability, total ... can aluminium pans be used on induction hobWeb9 apr. 2014 · Differentiation of HepaRG Progenitors Into Cholangiocytes HepaRG-HB were treated for 2 days with IL-6 (10 ng/ml), then for 2 days with sodium taurocholate hydrate (10 nM) and then for 2 days with sodium taurocholate hydrate (10 nM) and sodium butyrate (1.8 μM) to prevent spontaneous differentiation along the hepatocytic lineage. fisher price nativity toys