WebThe same procedure as described above was used for the experiment expressing Cdc53-3xHA in combination with Flag-Cdc34 or one of the Flag-Cdc34 derivatives, except that 5 μl of anti-HA antibody (0.4 mg/ml) (12CA5; Roche) was used for the immunoprecipitation and anti-HA HRP-conjugated antibody (3F10; Roche) was used for immunoblotting. WebDec 7, 2024 · OctA-Probe Antibody (H-5) is a mouse monoclonal IgG 1 κ OctA-Probe antibody, cited in 93 publications, provided at 200 µg/ml. raised against OctA (FLAG ® )-tagged proteins. OctA-Probe Antibody (H-5) is recommended for detection of OctA (FLAG®)-tagged fusion proteins of OctA and FLAG tagged proteins origin by WB, IP, IF …
DYKDDDDK Tag Antibody (Binds to same epitope as Sigma
WebThe coated protein is detected with HRP-conjugated anti-His tag antibody at concentration ranged from 1 to 0.008 µg/mL. A His-tagged recombinant protein is diluted in cell-culture medium. The diluted protein is loaded to 12% SDS PAGE gel in amount of 40, 20, and 10 ng. After electrophoresis, the His-tagged recombinant protein is detected with ... WebJan 1, 2010 · Horseradish peroxidase conjugated Mouse anti Histidine tag antibody clone AD1.1.0 (MCA1396P) ... As additional negative controls, two strips were incubated respectively with the anti-Flag antibody and the HRP-conjugated anti-mouse antibody, in order to monitor eventual non-specific signals due to the secondary antibodies used to … birth courses clearwater
Soluble Expression of Small Antibody Fragments against PD-L1 …
WebThe Sigma anti-FLAG M2 ( cat#F1804) is one of the best antibodies. I agreed with Kai's suggestion. You can dilute primary and secondary antibodies more than the standard dilution and give more ... WebI initially pulled down p53 using PAb421, and performed a Western Blot using an HRP-conjugated anti-FLAG antibody to detect if a certain POI was interacting with p53. WebJan 15, 2016 · Catalog Number: A FLAG-tagged construct was expressed in HEK293T cells and 50 µg of clarified lysate was loaded for SDS-PAGe followed by Western Blotting with a FLAG-HRP conjugated antibody. The goal was to test this antibody so that one can exclude secondary HRP conjugated antibody step and save one hr of incubation. daniels from scratch