WebBD Cytofix™ Fixation Buffer Can be used to fix unstained cells for subsequent immunofluorescent staining of intracellular cytokines. Can also be used to fix cells after immunofluorescent staining in order to preserve the light-scattering signals and fluorescent intensities of cells for analysis at a later time. Supplier: BD 554655 WebThis kit is commonly used for staining both cytoplasmic proteins as well as proteins in the secretory pathway such as chemokines and cytokines. It is not recommended for intranuclear staining. Instead, please refer to the Foxp3 Transcription Factor Buffer Set (cat. 00-5523) for nuclear staining.

PE Active Caspase-3 Apoptosis Kit - BD Biosciences

WebApr 11, 2024 · Then, cells were fixed and permeabilized using Cytofix/Cytoperm with Golgistop Kit (Cat No. 554715; BD biosciences pharmingen, US) according to manufacturer's instruction. After that, cells were incubated with fluorescently conjugated intracellular antibodies. WebResuspend cells in 100 µl of BD Cytofix/Cytoperm™ intracellular staining buffer (BD Cytofix/Cytoperm™ Kit, Cat. No. 554714) per well. 10. Incubate 30 minutes at room temperature. 11. Wash 2X with 200 µl of 1X Perm/Wash buffer (provided in the BD Cytofix/Cytoperm Kit) per well. Centrifuge at 250 x g for 5 minutes and aspirate … highest selling comics 1

PerCP-Cy™5.5 Armenian Hamster Anti-Mouse IL-9 — 561492

WebBD Cytofix/Cytoperm™ Fixation/Permeabilization Solution (Cat. No. 554714) is suitable for staining most cytokines and cell surface markers. This buffer system can also be used to stain some transcription factors … http://101.200.202.226/files/prod/manuals/201504/16/134554001.pdf WebCells were washed once in PBS, then fixed and permeabilized using the BD Cytofix/Cytoperm™ Kit (Cat. No. 554714) for 20 min at room temperature (RT), pelleted and washed with BD Perm/Wash™ buffer (component of Cat. No. 554714). Cells were subsequently stained with the FITC rabbit anti- active caspase-3 antibody (clone C92 … highest selling cheeses in america