WebNov 10, 1998 · The concentration of each streptavidin mutant was determined from A at 280 nm by using the extinction coefficient for a minimum-sized core streptavidin E 0.1% 280 nm = 3.55 . SDS/PAGE was carried out by using 15% polyacrylamide gels . Proteins were stained with Coomassie Brilliant Blue (Sigma). ... The biotin-binding affinities (K a) ... WebThe extinction coefficient of a 1% solution at 280 nm is 32. A high-affinity biotin binding protein. Packaging. 1 , 10 , 100 mg. 1 g. Warning. Toxicity: Standard Handling (A) Unit …
Streptavidin from S4762 - Sigma-Aldrich
WebOligo Calculation Tool. This tool calculates melting temperature (Tm), molecular weight (MW), Extinction Coefficient (OD/μmol, μg/OD) for oligos. Please input the oligo sequence in the text-box below. 1. Sequence (Paste the raw … WebAug 18, 2024 · Thus, the molar extinction coefficient can be obtained by calculating the slope of the absorbance vs. concentration plot. Since in most instances the path length b of the cuvette is equal to 1 cm, the slope is the same value as ε. This page titled Determining the molar extinction coefficient of gold nanoparticles is shared under a CC BY-NC-SA ... how does a primus stove work
Fluorescein biotin AAT Bioquest
WebBiotin quantitative labeling: biotin with optimized structure and chromophore group can be quantified after labeling. Essential Information Molecular weight 810.92 Excitation/Absorption maximum (nm) 354 Emission maximum (nm) 509 Molar extinction coefficient ε (L⋅mol−1⋅ cm−1) 29000 Correction coefficient of 280nm(CF280) 0.29 WebThis bifunctional Cy3 biotin conjugate demonstrates excellent avidin binding and strong fluorescence upon binding to a protein or nucleic acid target. It has avidin-binding … Webpercent solution extinction coefficients (i.e., one must convert from 10 mg/ml units to 1 mg/ml concentration units). (A / εpercent) 10 = concentration in mg/ml The relationship … how does a pringles pinhole camera work